The hippocampus receives input from and sends output to the rest of the brain via a structure known as the entorhinal cortex, which is located beneath the anterior (frontal) region of the hippocampus.
of the hippocampal formation: a review of anatomical data review. Finally, we will show some examples for the use of brain slices in further applications. Anatomy of the hippocampus The anatomy of the hippocampus is of chief importance to its function. Modern place field studies typically use a video camera to record the location of. Here, we provide a thorough protocol for the dissection, extraction, and acute horizontal slicing of the murine brain, and discuss challenges and potential pitfalls of this technique. In contrast to other slice preparations, horizontal slicing allows to keep the fibers of the hippocampal input path (perforant path) in a fully intact state within a slice, which facilitates the investigation of entorhinal-hippocampal interactions. Here, a detailed protocol for a successful preparation of vibratome-cut horizontal hippocampal slices of mouse brains is provided. Moreover, different cutting planes can be applied for vibratome brain slice preparations. Brain slice preparations with a vibratome allow a better preservation of the tissue structure and guarantee a sufficient oxygen supply during slicing, which present advantages over the traditional use of a tissue chopper. There exist several different ways of slice preparations. Therefore, brain slices represent an ideal model system to assess protein functions, to investigate pathophysiological processes involved in neurological disorders as well as for drug discovery purposes.
Hippocampal brain slices can be used for several different applications, including electrophysiological recordings, light microscopic measurements as well as several molecular biological and histochemical techniques. Brain slices are a frequently used methodology when exploring neurophysiological functions of the hippocampus. This connectivity is accomplished via different informational flows in the form of fiber pathways. The hippocampus receives a high degree of intra- and inter-connectivity, securing a proper communication with internal and external brain structures. The hippocampus is a highly organized structure in the brain that is a part of the limbic system and is involved in memory formation and consolidation as well as the manifestation of severe brain disorders, including Alzheimer's disease and epilepsy.
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